Description
GenJet™ DNA In Vitro Tranfection Reagent for 3LL cell is pre-optimized for transfecting 3LL cells. 3LL also called Lewis lung carcinoma was discovered by Dr. Margaret R. Lewis of the Wistar Institute in 1951. This tumor originated spontaneously as a carcinoma of the lung of a C57BL mouse. The tumor does not appear to be grossly hemorrhagic and the majority of the tumor tissue is a semifirm homogeneous mass.
Refer to the following optimal transfection conditions for maximal trans...
Description
GenJet™ DNA In Vitro Tranfection Reagent for NMuMG cells is pre-optimized for transfecting NMuMG cells. NMuMG is mouse mammary gland, normal epithelial. NMuMG cells express different integral surface proteoglycans (e.g. syndecan). They are used for studies of cell adhesion and growth factor-binding molecules as well as for analysis of growth factor effects on cell proliferation.
Refer to the following optimal transfection conditions for maximal transfection efficiency on NMuMG ce...
Contents Per Kit:
● 1×1.0 ml of GenJet™ DNA Transfection Reagent
● 1×1.0 ml (x50) of HapiCell™ Transfection Toxicity Removal Cocktail
Description
GenJet™ DNA In Vitro Tranfection Reagent for SMC is pre-optimized for transfecting smooth muscle cells. Smooth muscle cells are notoriously hard to transfect partly due to the transfection toxicity related cell death. In combination of our proprietary HapiCell™ Toxicity Removal Cocktail, the pre-optimized transfection reagent gave u...
Description
GenJet™ DNA In Vitro Tranfection Reagent for epithelial cell is pre-optimized for epithelial cells. By cross-linking a peptide ligand, this pre-optimized GenJet™ reagent specifically binds epithelial cells and give superior efficiency on epithelial cells.
Refer to the following optimal transfection conditions for maximal transfection efficiency on epithelial cells. GenJet™ reagent, 1.0 ml, is sufficient for 600 to 1200 transfections in 24 well plates or 300 to 600 transfection...
Description
GenJet™ DNA In Vitro Tranfection Reagent for L929 cell is pre-optimized for transfecting L929 cells. L929 is a murine aneuploid fibrosarcoma cell line which is often used to assay TNF-alpha and TNF-beta. Treatment with TNF initiates apoptosis and subsequent cell death. The level of detection is on the order of 50 pg/mL. Another cell line which is approximately 5-10-fold more sensitive is WEHI-164. Some L929 subclones have been developed that allow detection of TNF at concentrat...
Description
GenJet™ DNA In Vitro Tranfection Reagent for PC12 cell is pre-optimized for most efficiently transfecting PC12 cells. PC12 is a cell line derived from a pheochromocytoma of the rat adrenal medulla. PC12 cells stop dividing and terminally differentiate when treated with nerve growth factor. This makes PC12 cells useful as a model system for neuronal differentiation.
Refer to the following optimal transfection conditions for maximal transfection efficiency on PC12 cells. GenJet™ ...
Description:
GenMute™ siRNA Transfection Reagent for C2C12 Cell is pre-optimized for transfecting siRNA to C2C12 cells. C2C12 cells were originally obtained by Yaffe and Saxel (1977) through selective serial passage of myoblasts cultured from the thigh muscle of C3H mice 70 h after a crush injury. These cells were shown to be capable of differentiation. C2C12 cells are a useful model to study the differentiation of non-muscle cells to skeletal muscle cells (e.g myosin phosphorylation mechani...
Description:
GenMute™ siRNA Transfection Reagent for Hela Cell is pre-optimized for transfecting siRNA to Hela cells. GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 10 pmoles siRNA or miRNA mimics in 24-well plate.
Refer to the following optimal transfection conditions for maximal silencing on Hela cells. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.
Summary of Optimal Transfection Co...
Description:
GenMute™ siRNA Transfection Reagent for HepG2 Cell is pre-optimized for transfecting siRNA to HepG2 cells. HepG2 (Hepatocellular carcinoma, human) is a perpetual add cell line which was derived from the liver tissue of a 15 year old caucasian male with a well differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a model chromosome number of 55 and are not tumorigenic in nude mice. The cells secrete a variety of major plasma proteins e.g.. album...
Description:
GenMute™ siRNA Transfection Reagent for Insulinoma (INS) cell is pre-optimized for transfecting siRNA to INS. Insulinoma cell is a tumor cell line derived from pancreatic beta cells. Normal beta cells secrete insulin in response to increases in blood glucose. The resulting increase in insulin acts to lower blood glucose back to normal levels at which point further secretion of insulin is stopped. In contrast, the secretion of insulin by INS cell is not properly regulated by gluc...
