Description:
GenMute™ siRNA Transfection Reagent for Jurkat Cell is pre-optimized for transfecting siRNA to Jurkat cells. Jurkat cells are an immortalized line of T lymphocyte cells that are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV. Jurkat cells are also useful in science because of their ability to produce interleukin 2. Their primary use, however, is to determine the mechanism of di...
Description:
GenMute™ siRNA Transfection Reagent for MCF7 Cell is pre-optimized for transfecting siRNA to MCF7 cells. MCF-7 cell is a breast cancer cell line was isolated in 1970 from a 69-year-old Caucasian woman. This cell line retained several characteristics of differentiated mammary epithelium including the ability to process estradiol via cytoplasmic estrogen receptors and the capability of forming domes. This cell line expresses the oncogene Wnt7B. Tumor necrosis factor alpha (TNF alp...
Description:
GenMute™ siRNA Transfection Reagent for MEFs is pre-optimized for transfecting siRNA to Mouse Embryonic Fibroblasts (MEFs). MEFs are required to support the growth of undifferentiated mouse or human ES cells, iPS cells, thus they are also called feeder cells. MEFs are isolated from mouse embryos and are used at their early passages. Before MEFs are used as feeder cells, they must be treated by irradiation or mitomycin C to stop the cells from further dividing.
Refer to the follow...
Description:
GenMute™ siRNA Transfection Reagent for Primary Keratinocytes is pre-optimized for transfecting siRNA to primary keratinocytes. Primary keratinocytes are known for notorious resistance for DNA and siRNA transfections. With the pre-optimized transfection conditions, average 90% silencing can be obtained.
Size & content:
– GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 20 pmoles siRNA or miRNA mimics in 24-well plate
– GenMute™ Transfection Buffe...
Description:
GenMute™ siRNA Transfection Reagent for Primary Keratinocytes is pre-optimized for transfecting siRNA to primary keratinocytes. Primary keratinocytes are known for notorious resistance for DNA and siRNA transfections. With the pre-optimized transfection conditions, average 90% silencing can be obtained.
Size & content:
– GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 20 pmoles siRNA or miRNA mimics in 24-well plate
– GenMute™ Transfe...
Description:
GenMute™ siRNA Transfection Reagent for PMG is pre-optimized for transfecting siRNA to primary macrophages. Macrophages are white blood cells within tissues, produced by the differentiation of monocytes. Monocytes and macrophages are phagocytes, acting in both non-specific defense (innate immunity) as well as to help initiate specific defense mechanisms (adaptive immunity) of vertebrate animals. Their role is to phagocytose (engulf and then digest) cellular debris and pathogens ...
Description:
GenMute™ siRNA Transfection Reagent for Primary Neurons is pre-optimized for transfecting siRNA to primary neurons. Primary neurons are notoriously heard to transfect. With the pre-optimized siRNA transfection conditions, GenMute™ reagent was confirmed to give up to 90% silencing at final 50 nM siRNA on primary cultured neurons.
Size & content:
– GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 25 pmoles siRNA or miRNA mimics in 24-well p...
Description:
GenMute™ siRNA Transfection Reagent for RAW 264.7 Cell is pre-optimized for transfecting siRNA to RAW 264.7 cells. RAW 264.7 cells are a macrophage-like, Abelson leukemia virus transformed cell line derived from BALB/c mice. RAW 264.7 cell slide is often used for immunocytochemical analysis of protein and protein-protein interaction. Procedures for immunohistochemistry can be directly applied.
Size & content:
– GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions ba...
Description:
GenMute™
siRNA Transfection Reagent for SMC is pre-optimized for transfecting siRNA to
smooth muscle cells (SMC).
Refer to the following optimal transfection conditions for maximal silencing on
SMC. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or
~416 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Cell culture medium
Confluency of cell on the day of transfection
Optimal siRNA concentration
Diluent for siRNA an...
Description:
GenMute™ siRNA Transfection Reagent for U2OS Cell is pre-optimized for transfecting siRNA to U2OS cells. The U2OS cell line, originally known as the 2T line, was cultivated from the bone tissue of a fifteen-year-old human female suffering from osteosarcoma. Established in 1964, the original cells were taken from a moderately differentiated sarcoma of the tibia. U2OS cells exhibit epithelial adherent morphology and viruses were not detected in the line during co-cultivation with ...
