Adeno-associated virus (AAV) is a small virus (20 nm in diameters) replication-defective, nonenveloped virus, which infects humans and some other primate species. AAV is not currently known to cause disease and consequently the virus causes a very mild immune response. AAV can infect both dividing and non-dividing cells and may incorporate its genome into that of the host cell.
With our proprietary genetically engineered AAV·HT™ packaging cell, we are offering shRNA rAAV (conventional single stranded rAAV or double-stranded rAAV also called self-complementary rAAV*) production services for efficient silencing in vitro and in vivo.
If commercial shRNAs or siRNAs of your target gene are not available or non-functional, or your project requires special design for further development, we offer special shRNA design without additional charge!
Advantages: - One-stop service: from shRNA design to packaging. - High infection efficiency. - Quick turnaround time: 2 to 3 weeks. - Cost-effective. - Special design request acceptable, FREE!
Wide choice of rAAV cis vectors: Requirements: GenBank access #, sequence of gene of interest or shRNA is needed.
Deliverables: Total >1.0 ml at >1013 VG/ml** shRNA rAAV for large scale production.
With our proprietary genetically engineered AAV·HT™ packaging cell and a modified rAAV cis plasmid, the shRNA rAAV yield is easy to reach super high level--------total 1E+15 VG.
For super high level rAAV production up to 1E+15 VG , please contact us to request a quote.
* Some terms may apply for custom double-stranded or self-complementary rAAV production service. ** Final viral yield may depend on the transgene in the virus. For rAAV serotype 2, we guarantee final deliverable of >2.0 ml at >1E+12 VG/ml.
Features:
– Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells.
– Multiple serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9).
– Integration into chromosome 19 of host genome.
– Induce efficient gene silencing.
Wide Choice of AAV cis Vectors:
Service Description:
1. Synthesize and clone shRNA into AAV cis vectors.
2. Large scale transfection of AAV·HT™ 293 cells into 2xcell stack.
3. Harvest rAAV followed by puri...
Features:
– Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells.
– Multiple serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9).
– Integration into chromosome 19 of host genome.
– Induce efficient gene silencing.
Wide Choice of AAV cis Vectors:
Service Description:
1. Synthesize and clone shRNA into AAV cis vectors.
2. Pilot scale transfection of AAV·HT™ 293 cell into 10×150 mm plates.
3. Harvest rAAV followed ...
Features:
– Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells.
– Multiple serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9).
– Integration into chromosome 19 of host genome.
– Induce efficient gene silencing.
– Wide choice of AAV cis vectors (promoterless, CMV, CAG (also known as CBA), U6, H1, UBC, Synapsin promoters with or without reporter genes like GFP, mRFP, Luc, LacZ, etc).
Service Description:
– ...
Features:
– Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells.
– Multiple serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9).
– Integration into chromosome 19 of host genome.
– Induce efficient gene silencing.
– Wide choice of AAV cis vectors (promoterless, CMV, CAG (also known as CBA), U6, H1, UBC, Synapsin promoters with or without reporter genes like GFP, mRFP, Luc, LacZ, etc).
Service Description:
– ...
Cell-based shRNA Validation Service:
– Cloning cDNA of your targeted gene into a validation plasmid
– Design and synthesize ~6 shRNA oligoes followed by cloning them to a rAAV cis vector.
– Transient co-transfection cDNA/shRNA plasmids.
– Quantification of target gene silencing via qRT-PCR
Available shRNA Validation Platforms:
– Constitutive shRNA knockdown
– Inducible shRNA knockdown
Guaranteed Knockdown:
We guarantee the knockdown of >80% at mRNA leve...
