GenMute™ siRNA Transfection Reagent for Hela Cell

¥ 1,980.00

购买数量:

  • 目录号: SL100568-HELA
  • 包装: 1.0 ml

请选择:



Description:
GenMute™ siRNA Transfection Reagent for Hela Cell is pre-optimized for transfecting siRNA to Hela cells. GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 10 pmoles siRNA or miRNA mimics in 24-well plate.

Refer to the following optimal transfection conditions for maximal silencing on Hela cells. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:

Cell culture medium
Confluency of cell on the day of transfection
Optimal siRNA concentration
Diluent for siRNA and GenMute
Reagent
Incubation Time to Form GenMute™/siRNA Complex
Maximal Silencing Efficiency

Transfection Results:
Reporter Gene
Silencing Efficiency (% )

 


DMEM with 4.5 g/L glucose, 10% FBS

~50%
20.0 nM

1xGenMute™ Transfection Buffer
~15 minutes at RT
48 hours

 

Renilla Luciferase
90%


Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Data Sheet & Protocol




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