Product Category: recombinant AAV
Description: AAV9-Synapsin-GFP is the serotype 9 AAV which express eGFP under the Synapsin promoter which restricts transgene expression only in neurons.
Titer: 1×1012 ~ 1×1013 GC/ml
Size: 100 ul
Storage Buffer: PBS with 5% Glycerol
Infection Protocol:
Product Category: recombinant AAV, AAV9-ChIEF-tdTOMATO
Description:
AAV9-ChIEF-tdTOMATO the serotype 9 rAAV which expresses a hybrid of Channelrhodopsin-1/2 under CAG promoter with tdTOMATO tag.
ChIEF is a hybrid of channelrhodopsin 1 and 2 with additional L170I mutation (ChR1 numbering) or at L131I (ChR2-numbering), leading to large currents in oocytes and HEK-cells and almost wild type like open state life time. ChIEF function as light-gated ion channels and very useful for many bioenginee...
Product Category: recombinant AAV, AAV9-CMV-mRFP
Description:
AAV9-mRFP is the serotype 9 rAAV which expresses mRFP under the CMV promoter. AAV9-mRFP express a monomeric red fluorescent protein mutant from Discosoma (DsRed) , overcome the tetramerization which is toxic to cells. mRFP can be used as a reporter in target cells. Used as a control.
Titer: 1×1012 ~ 1×1013 GC/ml
Size: 200 ul
Storage Buffer: PBS with 5% Glycerol
Infection Protocol:
Product Name: AAV Serotypes Testing Kit
Product Category: recombinant AAV
Description: This kits contains AAV1-CMV-GFP, AAV2-CMV-GFP, AAV5-CMV-GFP, AAV6-CMV-GFP, AAV8-CMV-GFP and AAV9-CMV-GFP viral stocks. It can be used for testing the infection efficiency on different cell types to see which serotype works best.
Titer: 1E+12~E+13 GC/ml
Size: 200 µl of each of the viral stock
Storage Buffer: PBS
Infection Protocol:
Features:
– Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells.
– Multiple serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9).
– Integration into chromosome 19 of host genome.
– Induce efficient gene silencing.
Wide Choice of AAV cis Vectors:
Service Description:
1. Synthesize and clone shRNA into AAV cis vectors.
2. Large scale transfection of AAV·HT™ 293 cells into 2xcell stack.
3. Harvest rAAV followed by puri...
Features:
– Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells.
– Multiple serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9).
– Integration into chromosome 19 of host genome.
– Induce efficient gene silencing.
Wide Choice of AAV cis Vectors:
Service Description:
1. Synthesize and clone shRNA into AAV cis vectors.
2. Pilot scale transfection of AAV·HT™ 293 cell into 10×150 mm plates.
3. Harvest rAAV followed ...
Features:
– Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells.
– Multiple serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9).
– Integration into chromosome 19 of host genome.
– Induce efficient gene silencing.
– Wide choice of AAV cis vectors (promoterless, CMV, CAG (also known as CBA), U6, H1, UBC, Synapsin promoters with or without reporter genes like GFP, mRFP, Luc, LacZ, etc).
Service Description:
– ...
Features:
– Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells.
– Multiple serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9).
– Integration into chromosome 19 of host genome.
– Induce efficient gene silencing.
– Wide choice of AAV cis vectors (promoterless, CMV, CAG (also known as CBA), U6, H1, UBC, Synapsin promoters with or without reporter genes like GFP, mRFP, Luc, LacZ, etc).
Service Description:
– ...
Cell-based shRNA Validation Service:
– Cloning cDNA of your targeted gene into a validation plasmid
– Design and synthesize ~6 shRNA oligoes followed by cloning them to a rAAV cis vector.
– Transient co-transfection cDNA/shRNA plasmids.
– Quantification of target gene silencing via qRT-PCR
Available shRNA Validation Platforms:
– Constitutive shRNA knockdown
– Inducible shRNA knockdown
Guaranteed Knockdown:
We guarantee the knockdown of >80% at mRNA leve...
