AAV Production, Full Service, Large Scale


请询价

  • 目录号: SL100801
  • 包装: 1.0 ml (>1X1013 VG/ml)

Features:
- Nonpathogenic with least immune response and best suitable for in vivo application.
- Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells.
- Integration into chromosome 19 of host genome (for wild type AAV ONLY).
- Multiple serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9).

Wide Choice of AAV cis Vectors:


Service Description:
- Cloning your gene of interest (GOI) into AAV cis vector.
- Large-scale preparation of pAAV cis plasmid.
- Large scale transfection of
AAV·HT™ 293 cells into 2xcell stack.
- Harvest rAAV followed by purification via advanced 2xCsCl ultra-centrifugation to obtain clinical trial grade of rAAV vector*.
-
Desalting, filter sterilization, and AAV titration via qPCR.


Required Materials: A NCBI accession # or gene symbol or plasmid DNA carrying your gene of interest (GOI).

Turnaround Time:
Full Service: 2~3 weeks.

Deliverables:
>1.0 ml of super purified high titer at >1E+13 VG/ml** rAAV stock for both in vitro and in vivo studies, i.e., delivering your gene of interest to actively dividing and non-dividing mammalian cells in culture or injection into mice, etc.

We offer discount for new customer, please request a quote with us today.

Service Inquiry / Request a Quote

Super High Yield:
With our proprietary genetically engineered AAV·HT™ packaging cell and a advanced purification approach, the rAAV yield is easy to reach super high level--------total 1E+15 VG. For super high level rAAV production up to 1E+15 VG total, please contact us to request a quote.
 

 


* Figure 1. A comparison of purity and infectivity of rAAV vectors from different sources showing super purified and super infectious (close to clinical trial grade) rAAV vector prepared via our advanced double CsCl ultra-centrifugation approach. 

A. rAAV vectors (total 1E+9 VG per lane)  from different sources were resolved in SDS-PAGE followed by silver staining.  Lane 1: GMP manufactured rAAV vector from CHOP; Lane 2: rAAV prepared via our advanced 2xCsCl ultra-centrifugation approach; Lane 3: rAAV from Vector Core of BCM; Lane 4: rAAV from our competitor "V"; Lane 5: rAAV from our competitor "C"; Lane 6: Protein marker.
B. Super infectious rAAV vector prepared via advanced double CsCl ultra-centrifugation.  Left panel: rAAV9-GFP (total 5E+9 VG) from our competitor "V" injected to mouse eye; Right panel: rAAV-9-GFP (total 5E+9 VG) purified via advanced 2xCsCl ultra-centrifugation injected to mouse eye. 


* Figure 2. A comparison of infectivity of rAAV vectors from different sources showing super purified rAAV prepared via advanced double CsCl ultra-centrifugation approach is super infectious.  . 

rAAV1-GFP (total 2E+9 VG) from different sources were injected to mouse muscle tissue.  The GFP fluorescence was visualized 3 weeks post injection.  A. rAAV1-GFP from our competitor "V"; B. rAAV1-GFP from our pre-made rAAVs stock purified via advanced double CsCl ultra-centrifugation.  C. Quantification data showed that our super purified rAAV (bar 2) is ~ 9 times more infectious than that (bar 1) prepared via conventional CsCl ultra-centrifugation.  


** Final viral yield may depend on the nature of transgene.  For rAAV serotype 2, we guarantee final deliverable of >2.0 ml at >1E+12 VG/ml.




版权所有 © 2019 思科生物科技有限公司.