PolyJet™体外DNA转染试剂

价格: ¥1,380.00

购买数量:

  • 目录号: SL100688
  • 包装: 1.0 毫升

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产品介绍:
基于我们专有的高分子聚合物合成技术,PolyJet™体外DNA转染试剂是一种可生物降解的高分子聚合物的DNA转染试剂。它能对 HEK293, COS-7, NIH-3T3, HeLa, CHO细胞以及更广范围难转染的哺乳细胞,确保高效、可重复性的转染结果。室温下,PolyJet™ 试剂在一个非常低的浓度,能固定DNA在电泳过程中的迁移,并在5分钟之内形成转染复合物。该试剂的一个显著特征是在转染复合物内吞之后能快速完全降解掉,细胞毒性低。PolyJet™ 试剂,1.0ml,能在24孔板中足够完成600-1200次转染,或者在6孔板中足够完成300-600次转染,是用于各种常用细胞系和难以转染的哺乳动物细胞系的一个价格优惠的替代产品。



Figure 1. A Cartoon Showing Biodegradation of PolyJet™ DNA Transfection Reagent After Endocytosis of Transfection Complex

产品特点:
- 细胞内吞之后能生物降解
- 产生高滴度的病毒
- 对长DNA片段(>89kb)同样有效
- 对单个DNA片段的转染和多个DNA片段的共转染同样有效
- 产生高水平的重组蛋白
- 转染步骤简单
- 价格优惠,物美价廉

储存条件
40C储存。若储藏合适,产品的稳定性能保持24个月以上。


PolyJet™体外DNA转染试剂具有极广谱的转染活性。

细胞系

转染效率 (% GFP)

细胞系

转染效率 (% GFP)

McArdle 7777
Hep3D
SHEP
3T3-442A
COS-7
CV-1
D 407
DHD Pro.b
3LL
B16-F10
BAEC
BHK-21
Ca Ski
CaCo2
CHO
HCS-2/8
HEK-293
HeLa
HLMEC
H-MVEC
Huh-7D12
ATT20
SK-N-SH
C2C12
HepG2

65-70%
67-76%
68-71%
35%
85-90%
60%
70%
70%
80%
85%
51%
80%
88%
60%
88%
61%
86%
88%
72%
59%
72%
46%
29%
46%
72%

SAOS-2
SN56
MC3T3-E1
Primary melanocyte
K562
L929
MCF-7
MDCK
Neuro2A
NIH 3T3
PC12
SH-SY5Y
SiHa
SKOV3
Huh-7
IGROV1
DF-1, Chicken Embryonic Cell
6CSFMEo
WEHI 231
A549
LNCap
Prim. mouse keratinocyte
Prim. human skin fibroblast
Prim. human pre-adipocyte
Prim. mouse embry. fibroblast

58%
81%
80%
35%
38%
59%
68%
68%
86%
76%
50%
25%
60%
65%
70%
35%
50%
71%
26%
75%
75
29%

50%
32%
30%


实例说明PolyJet™体外DNA转染试剂对常见细胞系优异的转染效率。


PolyJet
转染试剂和lipofectamine Plus转染试剂对中国仓鼠卵巢(CHO)细胞转染效率的比较分别使用PolyJet (左图)和lipofectamine Plus (右图)将HA标记的β­微管蛋白cDNA转染入中国仓鼠卵巢细胞。异硫氰酸荧光素(FITC)标记的抗HA标记的抗体被用来结合HA-β­微管蛋白(绿色)DM1a抗体被用来检测内源性的β­微管蛋白,它连接着罗丹明标记的二抗(红色)探针。上述图片是由德克萨斯州休斯顿医学院(University of Texas at Houston Medical School)的Dr. Shang Yin友情提供的。


PolyJet
试剂和一款领先产品Lipofectamine 2000HEK293FT细胞染效率的比较分别使用PolyJet(左图)和Lipofectamine 2000(右图)将GFP载体(pEGFP­N3)转染入HEK­293FT细胞。转染24小时后通过尼康Eclipse荧光显微镜检查细胞的转染效率


PolyJet™ 试剂和市场主导品牌产品Lipofectamine 2000HepG2细胞转染效率的比较。分别使用PolyJet (左图)和Lipofectamine 2000(右图)将GFP载体(pEGFP­N3)转染入HepG2 细胞。转染24小时后通过尼康Eclipse荧光显微镜检查细胞的转染效率


PolyJet™和Fugene HDMDCK细胞染效率的比较。分别使用PolyJet™(左图)和Fugene HD (右图)按照生产制造商的科学实验步骤将绿色荧光蛋白(GFP)DNA转导入MDCK细胞。转染48小时后在荧光显微镜下观察GFP和DAPI染色。上面的图片是由NYU医学中心Ge Zhou博士友情提供的。


PolyJet™试剂和
市场主导品牌产品Lipofectamine 2000MDCK细胞转染效率的比较。MDCK细胞是难转染的细胞使用我们专有的细胞转染操作程序PolyJet™ (左图)能高达70% GFP阳性细胞对比Lipofectamine 2000 (右图)大约 5%的阳性效率。分别使用由PolyJet™ (左图)和Lipofectamine 2000 (右图)将GFP载体(pEGFP­N3)转染入MDCK细胞。转染36小时后,通过尼康Eclipse荧光显微镜检查转染效率


PolyJet
试剂同市场主导品牌产品之一Fugene HDLNCap细胞转染效率的比较。分别使用PolyJet (左图)和Fugene HD(右图)将GFP载体(pEGFP­N3)转染入LNCap细胞。转染24小时后,通过尼康Eclipse荧光显微镜检查转染效率


PolyJet
试剂对神经细胞系Neuro2A有较高的转染效率使用PolyJet体外DNA转染试剂将pEGFP­C1质粒转染入Neuro2A细胞。转染24小时后,通过尼康Eclipse荧光显微镜观察Neuro2A细胞的DIC影像(左)荧光影像(右)。


PolyJet DNA体外转染试剂和Lipofectamine 2000在原代小鼠皮肤成纤维细胞上的细胞毒性的比较。小鼠成纤维细胞与指定的转染试剂/pEGFP­C1 (DNA)混合物在无血清DMEM高糖培养基孵育4小时,然后用全血清培养基替代。转染24小时后,细胞的尼康Eclipse荧光显微镜DIC相位成像图。


转染试剂操作步骤及其使用指南
- 一般性哺乳动物细胞转染操作步骤

- 简易转染操作步骤(适合熟练操作者)
- 悬浮293和CHO细胞转染操作步骤

- 对难转哺乳动物细胞转染操作步骤
- 包装慢病毒转染操作步骤
-
包装重组腺相关病毒转染操作步骤
注意事项及转染窍门


PolyJet体外DNA转染试剂感兴趣?网上注册即可收到一份免费试用样品。上网注册请点击账户注册,输入您的邮寄地址后,请发送邮件至info@signagen-china.com
 


 

用户评价:

PolyJet Transfection Reagent worked equally as well as lipofectamine 2000, with little evidence of cell death on 293, PC-3 and 22RV1 cells. I will defiantly consider switching over.

-----Tiffany Wallace, Ph.D., June 9, 2009, NCI / NIH


I only did side by side with the testing sample (PolyJet) and Lipo2000 with GFP transfection on COS-7 cells. The result was very good. PolyJet was even better than L2K.

------Feng Qiao, Ph.D., Jun 12, 2009, NEI / NIH


I tested the sample of PolyJet on my NIH-3T3 mouse fibroblasts this weekend. The results were much better than Lipofecatmine LTX. I'm attaching a powerpoint slide with my results (I did not quantify the % transfection efficiency, but the pictures get the point across). I found that the protocol for difficult-to-transfect cell lines worked better than the standard protocol.

-----Stephanie Murphy, Ph.D., June 29, 2009, Dartmouth College


I had chance to try your product finally. It was great success. I used HeLa cells and got 10% transfection efficiency (<0.1% for Lipofectamine). Thank you! I was wondering if I also try GenJet™ Plus DNA In Vitro Transfection Reagent? According to your website, the reagent works better than regular PolyJet.

-------Yumi Uetake, Ph.D., August11, 2009, UMASS


I tested PolyJet and it looks great on MDCK. We placed order. Thank you!

-------Ge Zhou, Ph.D., August 12, 2009, NYU


We are happy to provide feedback. PolyJet worked very well for us in HepG2 cells, we got approximately 80% efficiency with pMAX GFP plasmid, by following the conditions in your suggested protocol. We ran a comparison with Lipofectamine, which only showed approximately 20-30% transfection efficiency. We are planning experiments and will be ordering more soon.

-------Emily Mcallister, September 3rd, 2009, PBRC

 



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